Group spokeswoman Paula Sherman says they're not just worried about the potential hazards of uranium mining on the environment and health of those who live in the area near Sharbot Lake.
She says they're also upset that government officials aren't consulting with native communities before allowing companies to mine on traditional lands.
Members of the Sharbot Lake and Ardoch Algonquin First Nations say they want a moratorium on uranium mining on the land.
Non-native residents in the area have also voiced their opposition to the project, some with makeshift signs along the highway saying "No uranium mine" and "Radioactive death."
Sherman says the group is still in talks with the mining company, Frontenac Ventures Corp., which has mineral claims covering an area of about 60 square kilometres in Frontenac County.